Histological analysis and identification of the gene responsible for a germ cell deficient mutant in medaka

2014 Fiscal Year Final Research Report

• Project/Area Number: 24570066
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Utsunomiya University
• Principal Investigator: MASARU Matsuda 宇都宮大学, バイオサイエンス教育研究センター, 教授 (20414013)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: メダカ / 性分化 / 生殖細胞

Outline of Final Research Achievements

A germ cell deficient mutant has no germ cells at 30 days after hatching stage. So, I analyzed when the germ cells were missing and I narrowed the chromosomal region located on the responsible gene. First, I searched recombinant progeny of the heterozygous mutant and the HNI strain, and found a few recombinants. Therefore, I mated heterozygous mutant with the Hd-rR strain and found 34 recombinants from 975 progeny. Since these recombinants were heterozygous, I performed mating experiment to obtain homozygous offsprings. Analyses of phenotypes of these homozygous offsprings narrowed the responsible region to 87 kb.

Free Research Field

発生遺伝学