Central regulatory system of reproduction by neuropeptides including kisspeptin.

2015 Fiscal Year Final Research Report

• Project/Area Number: 24570067
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: St. Marianna University School of Medicine (2015); The University of Tokyo (2012-2014)
• Principal Investigator: Akazome Yasuhisa 聖マリアンナ医科大学, 医学部, 講師 (50302807)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: キスペプチン / ニューロペプチドFF / NPFF / GPR74 / GPR147 / kiss1 / kiss2 / メダカ

Outline of Final Research Achievements

Kisspeptin plays pivotal roles in reproduction in mammals. However, its functions in non-mammalian species remain enigmatic. I therefore analyzed signal transduction of medaka (Olyzias latipes) GPR74, which reportedly receives kisspeptin in human, and GPR147, which share common ligands (NPFF/RFRP) with GPR74. At low concentrations, NPFF or RFRP reduced forskolin-induced CRE-luciferase activity in HEK293T cells expressing GPR74/GPR147 . However, at higher concentrations, NPFF/RFRP increased CRE-luciferase activity in these cells. Such a concentration-dependent biphasic responsiveness of GPR74/GPR147 to ligands may suggest that medaka GPR74 and GPR147 have an intrinsic ability of maintaining the cellular response within a certain range.

Free Research Field

神経生物学および比較内分泌学