Studies of action mechanisms of molecular machineries for maintaining activity of coenzyme B12-dependent radical enzymes

2015 Fiscal Year Final Research Report

• Project/Area Number: 24570161
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Okayama University
• Principal Investigator: Mori Koichi 岡山大学, 自然科学研究科, 助教 (50379715)
• Co-Investigator(Kenkyū-buntansha): TOBIMATSU Takamasa 岡山大学, 大学院自然科学研究科, 准教授 (30188768)
• Co-Investigator(Renkei-kenkyūsha): TORAYA Tetsuo 岡山大学, 名誉教授 (70026318)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: ビタミンB12 / 補酵素B12 / ラジカル触媒酵素 / 再活性化因子 / 再活性化蛋白質 / メチルマロン酸尿症 / B12アデノシル化酵素 / 酵素反応機構

Outline of Final Research Achievements

Vitamin B12 is biologically converted to the coenzyme form, and participate in enzyme reactions. Coenzyme B12-dependent enzymes catalyze enzymatically difficult reactions by radical mechanism. On the other hand, coenzyme B12-dependent enzymes tend to inactivate accompanying side reactions. In order to overcome such inactivation, several coenzyme B12-dependent enzymes have specific reactivating system for an inactivated enzyme. In human, a malfunction of a coenzyme B12-dependent enzyme leads to metabolic disorder. Also, inactivation of enzyme will be a serious problem when it applied to industrial production. Furthermore, since vitamin B12 is a rare compound, efficient use is desired.
In this research project, we studied on proteins that participate in reactivation of coenzyme B12-dependent enzymes and recycling of coenzyme B12 in order to elucidate systems that maintain catalytic activity of coenzyme B12-dependent radical enzymes.

Free Research Field

生化学