2014 Fiscal Year Final Research Report
Genetic approach on rotor and stator subunit interaction of sodium-tranlocating V-ATPase complex
| Project/Area Number |
24570162
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Ehime University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MURATA Takeshi 千葉大学, 理学部, 教授 (80415322)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ナトリウム / ＶーＡＴＰアーゼ / 腸球菌 / イオン / サブユニット |
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| Outline of Final Research Achievements |
Enterococcus hirae V-ATPase is an ion-transporting rotary enzyme that consists of nine kinds of subunits. In order to understand the molecular mechanism of this enzyme, it is essential to clarify the overall architecture and the interaction among these subunits. In this study, we succeeded in X-ray crystal structure analysis of the catalytic headpiece (V1-ATPase) and present ATP-hydrolytic rotatory mechanism of V1-ATPase. Furthermore, based on biochemical and surface plasmon resonance analyses on reconstitution of V1-ATPase complex and mutagenesis of V0 rotor ring subunit, the features of interaction among rotor and stator subunits of V-ATPase were revealed.
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| Free Research Field |
機能生物化学
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