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2014 Fiscal Year Final Research Report

PDE analysis

Research Project

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Project/Area Number 24580085
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied entomology
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

MIYAZAWA mitsuhiro  独立行政法人農業生物資源研究所, 昆虫機能研究開発ユニット, ユニット長 (90370684)

Co-Investigator(Kenkyū-buntansha) YAMAZAKI Toshimasa  独立行政法人農業生物資源研究所, 先端ゲノム研究センター, 生体分子研究ユニット長 (40360458)
ISHIDA Yoko  神戸大学, 理学(系)研究科(研究院), グローバルCOE研究員 (90509861)
Project Period (FY) 2012-04-01 – 2015-03-31
Keywordsフェロモン分解 / アルコールオキシダーゼ / アルコールデハイドロゲナーゼ
Outline of Final Research Achievements

Pheromone binding proteins (PBPs) and pheromone degradation enzymes (PDEs) play a part in chemical communication of insects. Although the PBP is unfolds widely, molecular mechanism analysis of PDE is still insufficient. In this study, we identified two estimated enzymes from the antenna of Japanese silkworm when it affected pheromone degradation. We purified these enzymes from approximately 3,000 antennae of the silkworm. However, the purest fraction separated by high resolution ion exchange chromatography contains several proteins in SDS-PAGE analysis. PDE from silkworm might be composed of various subunit.

Free Research Field

タンパク質科学

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Published: 2016-06-03  

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