2014 Fiscal Year Final Research Report
Analysis of taste-recognition mechanism through taste receptor
| Project/Area Number |
24580172
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Food science
|
|---|
| Research Institution | Nigata University of Phermacy and Applied Life Sciences (2014)
Yamagata University (2012-2013) |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | 甘味受容体 |
|---|
| Outline of Final Research Achievements |
Sweet taste is perceived through sweet-taste receptor, which is a membrane protein of taste cells. In this study, we tried to construct expression system of amino terminal domain (ATD) of human sweet-taste receptor, that is a heterodimer of hT1R2 and hT1R3, in order to analyze the interaction between sweet-taste receptor and sweeteners.
We succeeded to produce ATD of hT1R2 and hT1R3 fused with maltose-binding protein (MBP) in E.coli. We further analyzed hT1R3-ATD, since its expression level was higher than that of hT1R2-ATD. The protein was very unstable, and the molecular size was found to be much larger than the size expected.
|
| Free Research Field |
酵素学
|
