Study on the activation of drug-metabolizing phase II enzymes by the consumption of the kaempferol glycoside derived from horseradish leaves

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580182
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Kobe University
• Principal Investigator: HASHIMOTO TAKASHI 神戸大学, (連合)農学研究科(研究院), 准教授 (90362764)
• Co-Investigator(Renkei-kenkyūsha): MIZUNO Masashi 神戸大学, 大学院農学研究科, 教授
• Research Collaborator: IWAMI Shiho ; SHIMOAKI Tomohiro ; OKAMOTO Mayumi ; KANAZAWA Kazuki ; AIHARA Yoshiko
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 薬物代謝第Ⅱ相酵素 / ケンフェロール配糖体 / 西洋ワサビ葉 / ICRマウス / IQI/Jic(ICR)無菌マウス / 4-ヒドロキシフェニル酢酸 / ルチン / 3,4-ジヒドロキシフェニル酢酸

Outline of Final Research Achievements

The study with ICR mice and IQI/Jic(ICR) germ-free mice demonstrated that the intestinal flora was involved in the activated drug-metabolizing phase II enzymes by the consumption of 3-O-[2-O-(β-D-xylopyranosyl)-β-D-galactopyranosyl]-kaempferol derived from horseradish leaves. 4-Hydroxyphenylacetic acid, which is a catabolite from the kaempferol glycoside, played an important role in the activation of drug-metabolizing phase II enzymes.
The consumption of rutin, one of quercetin glycosides, also activated drug-metabolizing phase II enzymes in mice, and the active compound was 3,4-dihydroxyphenylacetic acid, which is a catabolite of rutin.

Free Research Field

栄養化学