Screening of DNA polymerase species selective inhibitors from nutrients and food components, and analysis of novel healthy functions

2015 Fiscal Year Final Research Report

• Project/Area Number: 24580205
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Shinshu University (2015); Kobe Gakuin University (2012-2014)
• Principal Investigator: MIZUSHINA Yoshiyuki 信州大学, 学術研究院農学系, 教授 (20307705)
• Co-Investigator(Kenkyū-buntansha): IRINO Yasuhiro 神戸大学, 医学研究科, 助教 (10415565)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 健康機能性食品 / DNA合成酵素 / 酵素阻害剤 / 抗炎症作用 / 抗がん作用 / マイクロアレイ解析 / ノックダウン解析 / 作用機序

Outline of Final Research Achievements

Some nutrients and food components are known to have various healthy functions. In this study, I established the in vitro screening method of mammalian DNA polymerase (pol) inhibitors, and screened them from nutrients and food components. Especially, I found a cerebroside (glucosyl ceramide, N-{1-[(β-D-Glucopyranosyloxy)methyl]-2-hydroxyheptadeca-3,7-dien-1-yl}-2-hydroxyhexadecanamide) and a steroidal glycoside (eleutheroside A) as mammalian pol lambda specific inhibitors from soybean (Glycine max L.), and beta-mangostin as a DNA replicative pols inhibitor from mangosteen (Garcinia mangostana L.). These inhibitors of pol lambda and DNA replicative pols were related to anti-inflammatory effect and anticancer effect, respectively.

Free Research Field

食品機能学