Start up study of in vitro cellulose biosynthesis system using ascidian CesA

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580239
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Wood science
• Research Institution: The University of Tokyo
• Principal Investigator: KIMURA SATOSHI 東京大学, 農学生命科学研究科, 助教 (00420224)
• Co-Investigator(Renkei-kenkyūsha): NAKASHIMA Keisuke 沖縄科学技術大学院大学, マリンゲノミックスユニット, 研究員 (10422924) ; WADA Masahisa 東京大学, 大学院農学生命科学研究科, 准教授 (40270897) ; IGARASHI Kiyohiko 東京大学, 大学院農学生命科学研究科, 准教授 (80345181)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: セルロース / ホヤ / セルロース合成酵素 / Pichia

Outline of Final Research Achievements

Considerable progress has been made in the study of cellulose biosynthesis in plants and bacteria. However, it has been revealed that cellulose biosynthesis is extremely complicated system, e.g. necessity of multiple synthase and several associated proteins. In this project, we focused ascidian cellulose synthases (CiCesA) expected to a new model organism and simple system for cellulose biosynthesis study, and constructed recombinant CiCesA protein by Pichia expression system for in vitro experiment system of cellulose biosynthesis.

Free Research Field

細胞生物学