Production of recombinant salmon insulin-like growth factor binding proteins

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580256
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: SHIMIZU Munetaka 北海道大学, 水産科学研究科(研究院), 講師 (90431337)
• Co-Investigator(Renkei-kenkyūsha): ANDO Hironori 新潟大学, 自然科学系, 教授 (60221743)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 成長 / サケ科魚類 / ホルモン / 組換え蛋白

Outline of Final Research Achievements

The present study dealt with production of functional recombinant insulin-like growth factor binding proteins (IGFBPs) that are important regulators of IGFs. We first established a primary pituitary cell culture for immature masu salmon. We next expressed recombinant IGFBP-1a and -1b using bacterial cell system. These recombinant IGFBPs were added to the pituitary cell culture to look at how the inhibitory action of IGF-I on GH release was modulated. The result suggests that although salmon IGFBP-1a and -1b were believed to be inhibitors of IGF-I actions, they actually enhanced the action of IGF-I on the pituitary. Availability of recombinant protein will be extremely useful to further analyze functions of fish IGFBPs.

Free Research Field

魚類生理学