Analysis of the virulence related genes on the fish pathogenic bacteria residing in the rotifer intestine

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580270
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General fisheries
• Research Institution: Nagasaki University
• Principal Investigator: SUGA Koushirou 長崎大学, 水産・環境科学総合研究科(水産), 准教授 (60569185)
• Co-Investigator(Renkei-kenkyūsha): KANAI Kinya 長崎大学, 水産・環境科学総合研究科(水産), 教授 (40145222)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 魚病細菌 / 動物プランクトン

Outline of Final Research Achievements

To clarify the pathogenic mechanism caused by oral administration of fish pathogenic bacteria via the rotifer Brachionus plicatilis in larval fish, that the present study investigated the relationship between the bacteria and B. plicatilis. The bacteria strains, Aeromonas hydrophila, Edwardsiella tarda, Photobacterium damuselae subsp. piscicida, Pseudomonas plecoglossicida, Vibrio anguillarum and Vibrio ichthyoenteri, used at this study were selected by Pulsed-field Gel Electrophoresis genotyping method. The results from the addition of the bacteria into the culture of B. plicatilis showed that 1.0E+4 cells / ml of the bacteria did not affect their life span, fecundity, and hatching rate of amictic eggs, and an individual rotifer contained 3.67E+2 CFU of bacteria in their intestine.

Free Research Field

魚病学