2014 Fiscal Year Final Research Report
Development of a novel technology to introduce signal transduction molecules into zebrafish embryo using membrane-translocating motif
| Project/Area Number |
24580308
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MASUHIRO Yoshikazu 日本大学, 生物資源科学部, 准教授 (00336083)
YABU Takeshi 日本大学, 生物資源科学部, 研究員 (00551756)
IMAMURA Shintarou 独立行政法人水産総合研究センター, 研究員 (80510007)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ゼブラフィッシュ / 細胞膜透過性ペプチド / シグナル分子 / キメラ蛋白質 / 形態形成 / 細胞分化 / 可視化 |
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| Outline of Final Research Achievements |
Membrane translocating motif (MTM) composes a signal peptide sequence of hydrophobic amino acid residues, AAVLLPVLLAAP. To develop a technology to introduce directly signal transducing molecules into zebrafish embryos, we generated a recombinant MTM-tagged p53 (MTM-p53) in E.coli and then examined the transcriptional activity of the purified MTM-p53 in vitro and in vivo.
we developed in here a novel transducing technology that is able to directly introduce to zebrafish and then to functionally act MTM-p53 protein . This provides a useful approach to understand the spatiotemporal regulatory mechanism(s) of signal transducing molecules in zebrafish.
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| Free Research Field |
分子細胞生物学
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