2015 Fiscal Year Final Research Report
Molecular mechanisms regulating formation of the embryo proper and the extraembryonic tissues by EGAM1 homeoproteins
| Project/Area Number |
24580413
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Akita Prefectural University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 繁殖 / ES細胞 / 初期胚 / 発生 / 転写因子 / 遺伝子発現 |
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| Outline of Final Research Achievements |
Recently, we found the structurally related homeoproteins in both preimplantation mouse embryos and mouse ES cells. To identify roles of EGAM1 homeoproteins on embryogenesis, mouse ES cells stably expressing respective EGAM1 homeoproteins were generated. Upon exogenous expression in mouse ES cells, these homeoproteins can act as positive or negative regulators of differentiation, cell growth, and induction of gene expression involved in specific cellular functions of differentiated cell types. EGAM1 homeoproteins not only affect the generation of progenitor cells that arise during early embryogenesis, but also the progression of terminal differentiation, such as cardiomyogenesis, in mouse ES cells. Because EGAM1 homeoproteins are localized to the nuclei of mouse ES cells, it is probable that these proteins act as transcription factors. These results suggest that EGAM1 homeoproteins are crucial for progression of embryogenesis.
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| Free Research Field |
動物分子生殖生理学
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