Comprehensive virus detection by high-performance homology search and deep sequencing

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580430
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Rakuno Gakuen University
• Principal Investigator: ENDOH Daiji 酪農学園大学, 獣医学群, 教授 (40168828)
• Co-Investigator(Kenkyū-buntansha): MIZUTANI Tetsuya 東京農工大学, 農学部, 教授 (70281681)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 次世代シーケンサー / 縮重プライマー / バイオインフォマティクス / 網羅的ウイルス検出 / 網羅的細菌検出 / 高速相同性検出 / プライマーの自動設計 / 糞便微生物解析

Outline of Final Research Achievements

On reports of the emerging viruses, requirement for comprehensive surveillance for viruses has been increased. In this study, we create computer program which design primers for comprehensive detection of viruses. The program automatically designed primers for amplifying 143,557 species (14 families) viruses and 19 genera of bacteria. We also create sequence analysis program for analyzing vast numbers of sequence reads with high-performance homology search. These programs and results suggest the ability of the combination of comprehensive amplification and next-generation sequencer.

Free Research Field

ウイルス学