Therapeutic application of microRNA for canine oral malignant melanoma.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580459
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Gifu University
• Principal Investigator: MORI Takashi 岐阜大学, 応用生物科学部, 准教授 (40402218)
• Co-Investigator(Kenkyū-buntansha): SAKAI Hiroki 岐阜大学, 応用生物科学部, 准教授 (40283288) ; NOGUCHI Shunsuke 山口大学, 獣医学部, 准教授 (10701295)
• Co-Investigator(Renkei-kenkyūsha): AKAO Yukihiro 岐阜大学, 連合創薬医薬医療情報研究科, 教授 (00222505)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: メラノーマ / マイクロRNA

Outline of Final Research Achievements

We demonstrated the antitumor effect in vitro and in vivo of miR-205 that was also chemically modified by benzene-pyridine and had altered passenger sequence. In in vitro experiments, transfection with the synthetic miR-205 (miR-205BP) significantly inhibited the growth of human melanoma cells. On the basis of the results of a luciferase activity assay, miR-205BP directly targeted E2F1, as did Pre-miR-205. However, miR-205BP was much more resistant to RNase than Pre-miR-205 in fetal bovine serum and to RNase in mice xenografted with human melanoma tissues. In addition, the intratumoral injection of miR-205BP exhibited a significant anti- tumor effect compared with the case of control miRNA or Pre-miR-205 in human melanoma cell-xenografted mice.

Free Research Field

獣医腫瘍学