Analysis of role of fructan exohydrolase genes in efficient use of wintering energy of wheat

2014 Fiscal Year Final Research Report

• Project/Area Number: 24580501
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: YOSHIDA Midori 独立行政法人農業・食品産業技術総合研究機構, 北海道農業研究センター・寒地作物研究領域, 主任研究員 (00355455)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: フルクタン / 糖代謝 / コムギ / 耐凍性 / 雪腐病抵抗性

Outline of Final Research Achievements

In autumn, winter wheat accumulates fructan, which is a kind of fructose-based oligo- and polysaccharide with high hydrophilicity, and the content of fructan in wheat is associated with both freezing tolerance and snow mold resistance. Recently, we cloned several kinds of genes encoding FEH (fructan exohydrolase) from wheat, and analyses of their substrate specificities revealed that various FEHs, including 1-FEH, 6-FEH, 6-KEH (kestose exohydrolase) and 6&1-FEH, exist in wheat plants. Analysis of gene expression of these FEHs during a wintering period showed that seasonal changes in mRNA levels were different among genes. Seasonal changes and varietal difference in expressions of FEH genes might also play an important role in tolerance to environmental stresses during wintering.

Free Research Field

植物生理学