Development of auxiliaries for ligation of peptides at a site other than cystein

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590010
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Chemical pharmacy
• Research Institution: The University of Tokushima
• Principal Investigator: SHIGENAGA Akira 徳島大学, 大学院ヘルスバイオサイエンス研究部, 講師 (10423394)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: Native Chemical Ligation / フラグメント縮合 / タンパク質化学合成 / 光解離性補助基

Outline of Final Research Achievements

In this study, development of auxiliaries for ligation of peptides at a site other than cystein was challenged. The auxiliary that can be removed by UV-irradiation was syntheseized and was incorporated onto a side chain of asparagine (Asn). Ligation of peptides at Xaa-Asn site was successfully achieved by the use of the Asn derivative via auxiliary assisted ligation followed by photo-removal of the auxiliary. Application of the auxiliary to amino acids other than Asn is in progress.

Free Research Field

ペプチド化学