Development of phosphate affinity electrophoresis system for phosphorylation profiling of proteins with a wide range of molecular masses.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590050
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Hiroshima University
• Principal Investigator: KINOSHITA Emiko 広島大学, 医歯薬保健学研究院, 助教 (40379912)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: リン酸基親和性電気泳動 / Phos-tag

Outline of Final Research Achievements

On the basis of the principal of our original phosphate affinity electrophoresis technology (Phos-tag SDS-PAGE) , we have developed a system that permits to separate various cellular proteins with a wide range of molecular masses. A Bis-Tris-buffered gel was optimum for the analysis of phosphoproteins with molecular masses of less than 200kDa. For analyzing high-molecular-mass proteins with more than 200 kDa, on the other hand, a Tris-AcOH-buffered gel was optimum. We are now investigating phosphorylation states of a wide variety of cellular proteins using this system and are in the process of accumulating data to be open to the public on our Web site.

Free Research Field

物理系薬学