The development study of highly sensitive chemiluminescent probe for tumor hypoxia in vivo imaging

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590054
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: OKUDA Kensuke 岐阜薬科大学, 薬学部, 准教授 (00311796)
• Co-Investigator(Kenkyū-buntansha): NAGASAWA Hideko 岐阜薬科大学, 薬学部, 教授 (90207994)
• Co-Investigator(Renkei-kenkyūsha): KONDO Shinae 東京工業大学, 生命理工学研究科, 教授 (40314182)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 化学発光 / 分析科学 / 低酸素 / イメージング / 高感度 / 低侵襲性

Outline of Final Research Achievements

Chemiluminescence imaging has the advantage of lower detection limits and improved signal-to-noise ratio compared to existing fluorescent imaging. To visualize the hypoxic status of various tumor cells which are considered to be closely related to malignancy, I developed a probe that emit chemiluminescence, which is suitable for non-invasive bioimaging, under hypoxia. To utilize hypoxia selective reductive metabolic reaction, I designed and synthesized chemiluminescent probe molecules rationally. Then, I evaluated them using nitroreductase to select the molecule to emit luminescence from the reaction. Next, I analyzed reaction products to show the molecule worked as the enzyme substrate to emit luminescence. As a result, I obtained significant expertise to apply the molecule for in vivo chemiluminescent imaging.

Free Research Field

医歯薬学、有機化学、創薬化学