2015 Fiscal Year Final Research Report
Identification of the molecular target to regulate Cell Polarity during 3 dimensional morphogenesis.
Project/Area Number |
24590237
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General anatomy (including Histology/Embryology)
|
Research Institution | Yokohama City University |
Principal Investigator |
|
Research Collaborator |
OHNO Shigeo
MORIYAMA Kayano
ASADA Kio
FUKUDA Ayano
|
Project Period (FY) |
2012-04-01 – 2016-03-31
|
Keywords | 細胞極性 / 平面極性 / 形態形成 / 遺伝子相互作用 / aPKC / Daam1 / Wnt-PCP |
Outline of Final Research Achievements |
Proper regulation of cell polarity and directional cell movements is essential to form correct three-dimensional architecture. Atypical protein kinase C (aPKC) is known as the key regulator for apical-basal (z axis) cell polarity, however, it is not clarified the role in the regulation of cell sheet (x-y plane) polarity, as well as planar cell polarity (PCP) in relation to Wnts and other signaling cascades. In this study, we show that aPKC genetically connect to Daam1 (Dshevelled associated activator of morphogenesis 1), which plays important role in Wnt-PCP signaling. We also found functional interaction between aPKC and Daam1 in Xenopus embryogenesis. Further, we present physical interaction between aPKC and Daam1. These results strongly suggest that aPKC contributes planar cell polarity regulation, in vivo and in vitro.
|
Free Research Field |
分子細胞生物学、発生生物学、形態形成学
|