Morphological and functional changes of mitochondria through estrogen receptors during liver regeneration

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590255
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: University of Miyazaki
• Principal Investigator: HISHIKAWA Yoshitaka 宮崎大学, 医学部, 教授 (60304276)
• Co-Investigator(Kenkyū-buntansha): HINO Shin-ichiro 宮崎大学, 医学部, 講師 (00372699) ; TAKAHASHI Nobuyasu 宮崎大学, 医学部, 助教 (20404436)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ミトコンドリア / エストロゲン / 肝切除 / 肝再生 / 細胞増殖 / エストロゲン受容体 / ラット

Outline of Final Research Achievements

The aim of this study is to evaluate the morphological and functional changes of mitochondria through estrogen and its receptors during liver regeneration. We used for 70% partial hepatectomized rat samples to perform the molecular histochemical analysis including immunohistochemistry, western blot, and PCR. As a result, the cell proliferating activity was the peak at 48 hours after partial hepatectomy. At the same period, estrogen receptor alpha, Tfam (mitochondria related gene) and LinA (specific stem cell marker in liver regeneration) were also expressed in the regenerative liver tissues. Therefore, estrogen receptor alpha may be involved in the mitochondrial function during liver regeneration.

Free Research Field

分子組織細胞化学