Functional analysis of acid-sensitive ion channels in mouse pituitary gland.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590281
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Nagoya City University
• Principal Investigator: UEDA Takashi 名古屋市立大学, 医学(系)研究科(研究院), 准教授 (90244540)
• Co-Investigator(Kenkyū-buntansha): UGAWA Shinya 名古屋市立大学, 大学院医学研究科, 教授 (20326135)
• Co-Investigator(Renkei-kenkyūsha): SHIMADA Shoichi 大阪大学, 大学院医学系研究科, 教授 (20216063)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 刺激分泌関連 / イオンチャネル型オーファン受容体 / 酸感受性イオンチャネル / 下垂体後葉 / 傍分泌

Outline of Final Research Achievements

In mouse pituitary gland, the anterior (AL) and intermediate lobes (IL) expressed acid-sensing ion channel 1(ASIC1) and ASIC4, whereas the posterior lobe (PL) expressed transient receptor potential vanilloid type 2 (TRPV2) channel. TRPV2, not TRPV4, was expressed in a unique subpopulation of glia-like pituicytes in the PL. Proton activated ASIC4 as well as ASIC1 in Xenopus oocytes. A TRPV2 agonist probenecid elevated the intracellular calcium concentration in a subset of the isolated pituitary gland cells (approximately 12 % of total cells) and evoked ATP release in these cells. Since ATP is known to be a paracrine modulator of vasopressin release in the PL, TRPV2 may be involved in the regulation of the endocrine system.

Free Research Field

医歯薬学