2014 Fiscal Year Final Research Report
Identification of conversion of cells by proteomics analysis
| Project/Area Number |
24590353
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 質量分析 / 幹細胞 / iPS細胞 / 定量 |
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| Outline of Final Research Achievements |
We have been studying for the purpose of capturing the expression changes of proteins during the conversion process of the cell. We proceeded improvements in identification techniques for proteins using a mass spectrometer (MS). First, using a meter-long monolithic column in order to improve the separation of the sample. Second, cells were fractionated into the cell membrane, cytoplasm, nuclear. We were able to achieve an increase in the number of protein that can be finally identified. Furthermore, by combining with labeling for quantitative comparing between samples, it was possible to carry out the establishment of accurately quantifying technology less samples with high sensitivity. Was subjected to analysis of human iPS cells and the original fibroblasts using this technique and have enabled the identification of gene which identification was difficult until now.
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| Free Research Field |
幹細胞生物学
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