Screening for the genes which shows synergistic phenotype with MYCN-With the aim of new molecular target of neuroblastoma

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590376
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: MURAKAMI-TONAMI Yuko 愛知県がんセンター(研究所), 分子腫瘍学部, 主任研究員 (70405174)
• Co-Investigator(Kenkyū-buntansha): KADOMATSU Kenji 名古屋大学, 大学院医学系研究科・生物化学講座分子生物学, 教授 (80204519)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 神経芽腫 / 合成致死 / DNA損傷修復 / MYCN

Outline of Final Research Achievements

We identified SMC2, a subunit of condensin complex, as a candidate gene which shows synergistic lethal phenotype with MYCN amplification/overexpression. In addition, we found SMC2 regulates expression of DNA repair genes cooperation with MYCN (Cell Cycle, 13 (7) : 1115-31, 2014). To find further candidate genes, we screened for the genes which showed synthetic lethal phenotype with MYCN overexpression using shRNA library in neuroblastoma cells. We also improved the method of discovering combinational interactions using survival data (Bioinformatics, 29 (23) : 3053-59, 2013).

Free Research Field

細胞周期