2014 Fiscal Year Final Research Report
Immunological analysis of treatment resistant cancer stem cells
| Project/Area Number |
24590449
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | がん幹細胞 / 免疫療法 / がん抗原 / がん精巣抗原 |
|---|
| Outline of Final Research Achievements |
In this research period, we isolated cancer stem-like cells(CSCs)/cancer-initiating cells(CICs) from human lung, colon and kidney cancer line cells. To identify CSC/CIC-specific genes, we performed cDNA microarray analysis and found that numbers of cancer-testis (CT) antigens including OR7C1, DNAJB8 and SMCP were preferentially expressed in CSCs/CICs. Furthremore, we identified HLA-A24 restricted antigenic peptides encoded by amino acid sequences of OR7C1 and DNAJB8. Cytotoxic T lymphocytes (CTLs) specific for OR7C1 peptide and DNAJB8 peptide showed preferential cytotoxicity for CSCs/CICs compared with that of non-CSCs/CICs. These observations indicate that CT antigens-specific cancer immunotherapy might be promising approach for targeting treatment resistant CSCs/CICs.
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| Free Research Field |
実験病理
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