2015 Fiscal Year Final Research Report
Biological analysis of miR-210 which is deregulated in clear cell renal cell carcinoma.
| Project/Area Number |
24590485
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Oita University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | microRNA / トランスジェニックマウス / 腎臓 |
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| Outline of Final Research Achievements |
Previously, I have reported that miR-210 is constitutively upregulated in clear cell renal cell carcinoma because of the deregulation of VHL-HIF axis. To elucidate whether the upregulation of miR-210 is involved in the tumorigenesis and/or progression of renal cell carcinoma, I addressed the following two issues, 1) the identification of the target genes of miR-210 and 2) the observation of biological effect of miR-210 upregulation on renal tubules in vivo.
Research results; A miR-210 target gene which is conserved as target between human and mouse was identified by microarray analysis. MiR-210 transgenic mice which express miR-210 in renal proximal tubules were established and histopathologically analyzed. Tg-mice kept alive without specific symptoms and development of renal carcinoma at least for 96 weeks. The chronic inflammations were observed in kidneys of young Tg-mice, and their renal function was diminished with aging. The overexpression of miR-210 may damage the renal tubules.
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| Free Research Field |
実験病理学
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