Cross-talk regulation of viral replication and fatty acid synthesis by miRNAs

2015 Fiscal Year Final Research Report

• Project/Area Number: 24590565
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: MUNAKATA Tsubasa 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主席研究員 (50420237)
• Research Collaborator: KOHARA Michinori 東京都医学総合研究所, ゲノム医科学研究分野, プロジェクトリーダー (10250218) ; SAITO Makoto 東京都医学総合研究所, ゲノム医科学研究分野, 主任研究員 (20433021)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: マイクロＲＮＡ / 脂肪酸合成酵素 / Ｃ型肝炎ウイルス

Outline of Final Research Achievements

It is reported that miR-199a-3p represses HCV replication by targeting 5’ untranslated region (UTR) of HCV genomic RNA. On the other hand, induction of the lipogenesis pathway upon HCV infection is known to play an important role in regulating HCV propagation and pathogenesis. We have reported that fatty acid synthase (FASN), an enzyme essential for de novo synthesis of a fatty acid, was induced by HCV infection and replication, and that FASN was needed for the efficient replication of HCV. Moreover, we have found that there is a functional interaction between HCV and FASN through miR-199a-5p and -3p. Interestingly, expression of miR-199a-5p and -3p is regulated by HCV replication. These data demonstrate that miR-199a-3p simultaneously represses HCV replication and FASN expression, and that there exists a crosstalk between HCV replication and host lipogenesis at the level of miRNA-mediated regulation.

Free Research Field

ウイルス学