Development of the luciferase immunoprecipitation systems with high detection densitivity for anti-AQP4 antibody in neuromyelitis optica

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590720
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Nagasaki Kawatana Medical Center
• Principal Investigator: HIGUCHI osamu 独立行政法人国立病院機構長崎川棚医療センター(臨床研究部), その他部局等, 研究員 (50361720)
• Co-Investigator(Renkei-kenkyūsha): MATSUO Hidenori 長崎川棚医療センター, 副院長 (20380975)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: NMO / 自己抗体 / AQP4 / ルシフェラーゼ / LIPS

Outline of Final Research Achievements

Anti-AQP4 antibody in patient serum with neuromyelitis optica (NMO) is thought to be one of key molecules in the molecular pathogenesis of NMO. In addition, the quick inspection of the antibody concerned is an important matter in treatment strategy development of NMO, and the inspection system which is high-speed for high sensitivity is essential. Reliability of cell-based assay (CBA) which made full use of a fluorescent microscope system was the highest in the law conventionally, but problems about complexity and the quantifiability in the maneuver of the inspection had it pointed out. In this study, CBA problems include clear all, and, reliability was also succeeded in building a fully collateral has been innovative antibody test system.

Free Research Field

神経分子病態学