Evaluation for neurodevelopmental deficit by nanomaterials

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590774
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hygiene
• Research Institution: National Institute for Environmental Studies
• Principal Investigator: ISHIDO Masami 独立行政法人国立環境研究所, 環境リスク研究センター, 主任研究員 (60211728)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 銀ナノ粒子 / 神経発生毒性

Outline of Final Research Achievements

We first analyzed the particle size of the AgNP used in this study by DLS . The maximum peak in DLS analysis was observed at 10 nm. We applied a neurosphere assay for neurodevelopmental effects of AgNPs. AgNPs inhibited cell migration in a dose-dependent manner. There was a linear correlation between the inhibition of migration and the logarithm of the particle concentration . Preceding migrated cells were retarded and/or collapsed by exposure to AgNPs: lower doses of AgNPs (0.31～1.25 μg/ml) caused a 42% retardation for 48 h, while higher doses of AgNPs (2.5～10 μg/ml) collasped migrating cells. Furthermore, collapsed cells were TUNEL-positive and showed a defect in the mitochondrial membrane potential.
We also examined the effects of AgNPs on rat behavior. A single oral administration of bisphenol A into 5-day-old male Wistar rats caused significant hyperactivity. It was about 1.3～1.4 fold more active in the nocturnal phase than control rats.

Free Research Field

環境神経科学