Analysis of relationship between genotypes and inhabit environment of Legionella spp. detected by amoebic co-culture method

2016 Fiscal Year Final Research Report

• Project/Area Number: 24590841
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Public health/Health science
• Research Institution: Osaka Prefectural Institute of Public Health
• Principal Investigator: EDAGAWA AKIKO 大阪府立公衆衛生研究所, 衛生化学部, 主任研究員 (80321941)
• Project Period (FY): 2012-04-01 – 2017-03-31
• Keywords: レジオネラ / アカントアメーバ / アメーバ共培養法 / co-culture / 培養不能菌 / VBNC / LLAP

Outline of Final Research Achievements

In this study we performed amoebic co-culture method to investigate legionella contamination of environmental water samples. Of 120 water samples, 75.1% (91 samples) were positive for Legionella spp. by amoebic co-culture method combined with real-time PCR method. we performed Sequence-Based Typing to analyze relationship between genotypes and inhibit environment of L. pneumophila detected by amoebic co-culture method and mip gene PCR, however we could not obtain positive data. Among the 91 positive samples, 17 samples (14.2%) showed over 10% increase of bacteria numbers. Following sequence analysis found LLAP type such as L. lytica from the positive samples. Our results suggested that Legionella spp. including viable but non-culturable form highly inhabited environmental water.

Free Research Field

環境微生物