Development of rapid,multi-targetteddetection method for microbial pathogens and application for public health

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590843
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Public health/Health science
• Research Institution: Osaka Prefectural Institute of Public Health
• Principal Investigator: TAKAHASHI KAZUO 大阪府立公衆衛生研究所, その他部局等, その他 (10171472)
• Co-Investigator(Kenkyū-buntansha): SAKON Naomi 大阪府立公衆衛生研究所, 感染症部ウイルス課, 主任研究員 (50291216) ; KURATA Takako 大阪府立公衆衛生研究所, 感染症部ウイルス課, 主任研究員 (70435890)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 起因微生物 / 迅速 / PCR法 / 網羅的 / Qプローブ法 / 感染症

Outline of Final Research Achievements

We developed more rapid(within 1 hour including DNA extraction), highly sensitive,multipathogen-targetted detection methods (referred to Q probe method) against representative pathogens of infectious diseases. The Q-probe method for pertussis revealed almost equivalent quality with more rapid performance compared to a conventional LAMP (Loop-Mediated Isothermal Amplification)method. The Q-probe method for mycoplasma infection revealed that 72% of M.pneumoniae was defined within 1 h to be resistant to macrolide type of antibiotics, suggesting that this more rapid method confers shortening the treatment period. The Q-probe method for campylobacter also revealed its superior quality with more rapid performance compared to a conventional culture isolation method.

Free Research Field

感染症学　ウイルス免疫学