Development of high-sensitive and fast drug screening systems in body materials for investigation on cause of death

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590872
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Fukuoka University
• Principal Investigator: HARA Kenji 福岡大学, 医学部, 講師 (00090738)
• Co-Investigator(Kenkyū-buntansha): WATERS Brian 福岡大学, 医学部, 助教 (00609480) ; KUBO Shin-ichi 福岡大学, 医学部, 教授 (10205122) ; KASHIWAGI Masayuki 福岡大学, 医学部, 准教授 (70301687) ; MATSUSUE Aya 福岡大学, 医学部, 講師 (70309920)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 法医中毒学 / 体組織試料 / 試料処理 / 薬物スクリーニング / GC-MS / LC-MS/MS / 迅速 / 簡素化

Outline of Final Research Achievements

In the investigation of the cause of death in forensic toxicology, analysis of the drug is important. We have examined the development of screening methods for searching for a wide variety of drugs. Postmortem samples, since often contains a large amount of lipids, removal of lipids were mainly studied. Preparation of postmortem sample was studied on ion pair acetonitrile extraction, lipid removal by a specific filter and by organic solvent washing, and ion exchange purification. Fast GC-MS by use of two connected column, was able to apply to the measurement of rapid and sensitive. Except highly denatured sample, the methods developed in this study were feasible for practical applications. In this sample preparation, a wide range of from lipophilic drugs to hydrophilic drugs can be measured by a combination of GC-MS and LC-MS / MS.

Free Research Field

法医学