Investigation to clarify the role of endothelial mesenchymal transition in cardiac fibrosis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591113
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Mie University
• Principal Investigator: OKAMOTO Ryuji 三重大学, 医学部附属病院, 講師 (60378346)
• Co-Investigator(Kenkyū-buntansha): ITO Masaaki 三重大学, 大学院医学研究科・循環器内科学, 教授 (00223181)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 心臓線維化 / 心肥大 / 心筋梗塞 / 内皮間葉以降 / レポーターマウス

Outline of Final Research Achievements

We assessed the hypothesis that endothelial mesenchymal transition played an important role in cardiac fibrosis. We generated pTie2-Cre and lox-tdTomato double transgenic mouse with a marker for endothelial cell-derived cells and performed the ligation of left coronary artery. Additionally we crossed those mice with pFSP1-EGFP mice. tdTomato expression was recognized specifically in endothelial cells. In cardiac fibrosis some cells expressed both the tdTomato and EGFP. However we could not observe the cells expressing tdTomato and periostin, a fibroblast-specific marker, simultaneously. Therefore we concluded endothelial mesenchymal transition did not play an important role in cardiac fibrosis.

Free Research Field

心肥大、高血圧症