Class-switching of immunoglobulins induced by pneumococcal vaccine

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591161
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: TAKAFUMI Suda 浜松医科大学, 医学部, 教授 (30291397)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Yutaro 浜松医科大学, 医学部, 助教 (60436962) ; NAGATA Toshi 浜松医科大学, 医学部, 教授 (90275024)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 肺炎球菌 / ワクチン / 樹状細胞

Outline of Final Research Achievements

Theoretically, pneumococcal polysaccharide vaccine with 23 serotypes of capsular polysaccharide (PPV23) can induce only serotype-specific IgM antibody. In the present study, we showed that PPV23 readily induced both IgG- and IgA- serotype-specific antibodies through immunoglobulin class-switch recombination (CSR) in mouse and human. Next, we demonstrated that CD11b+ lung dendritic cells (DC), but not CD103+ lung DC, elicited T-cell independent CSR in the presence of toll-like receptor (TLR) ligands plus certain cytokines, such as IL-6 and IL-10. These results suggest that PPV23 induces protective serotype-specific IgG and IgA antibodies through an interaction of particular phenotypes of DC with IL-6 and IL-10.

Free Research Field

呼吸器内科