Generation of kidney by interspecific blastocyst injection of pluripotent stem cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591209
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Yokohama City University
• Principal Investigator: HASHIMOTO Tatsuo 横浜市立大学, 医学(系)研究科(研究院), 客員研究員 (20363806)
• Research Collaborator: UMEMURA Satoshi 横浜市立大学, 医学研究科, 教授 ; SUZUKI Syota 横浜市立大学, 大学院医学研究科 ; NAKAUCHI Hiromitsu 東京大学, 医科学研究所, 教授 ; YAMAGUCHI Tomoyuki 東京大学, 医科学研究所, 特任准教授 ; PENNINGER Josef オーストリア科学アカデミー, 分子生物工学研究所, 教授 ; NAKAZAWA Masatoshi 横浜市立大学, 医学部, 准教授
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 腎臓再生

Outline of Final Research Achievements

The aim of this study is to generate rat kidney in mouse by inter specific injection of stem cells, toward human kidney generation. At first, I injected mouse ES cells to generate mouse kidney.
First, I generated kidney deficient mouse. Second, I injected mouse ES cells into embryo. Kidney deficient embryo would be expected to exist 25 % under the Mendelian ratio. Rare mouse which has fully functioned kidney from injected cells can alive. An advice from coworker helped me to mix background. Then I can get more mice than before.
This study has possibility to generate brand new medical treatment towards human organ generation.

Free Research Field

腎臓内科学