Pathopysiology of fuluminant type 1 diabetes and development of its new therapy

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591320
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: University of Yamanashi
• Principal Investigator: ENDO Toyoshi 山梨大学, 総合研究部, その他 (00152017)
• Co-Investigator(Kenkyū-buntansha): KANESHIGE Masahiro 山梨大学, 総合研究部, 助教 (20377518) ; KOBAYASHI Tetsuro 公益財団法人冲中記念成人病研究所, 研究室, 研究員 (30113442) ; TANAKA Syoichiro 山梨大学, 総合研究部, 助教 (70377521) ; TAKIZAWA Soichiro 山梨大学, 総合研究部, 助教 (80456467)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: アミラーゼ / 自己免疫性膵炎 / 劇症１型糖尿病 / ２型糖尿病 / ELISA

Outline of Final Research Achievements

To study epitopes of the autoantibody against AMY, we performed an ELISA using synthetic peptides corresponding to the C-terminal of AMY as antigens, and found that peptide 7 (P7) showed antigenicity to the autoantibody.Using P7 as an antigen, we screened for the autoantibody in patients with pancreas diseases. we screened for AMY autoantibody in AIP using the P7-coated ELISA plate (P7-ELISA). Eighteen out of 32 patients with AIP from our cohort were positive, suggesting that the results of P7-ELISA were more specific than those with C-AMY-ELISA. Of the 86 T2DM patients, 14 (16%) patients were positive for AMY autoantibodies. Fasting serum C-peptide levels of antibody positive-T2DM patients were significantly lower than that of antibody negative patients.
We immunized P7 peptide into subcutaneous tissues of mouse biweekly.

Free Research Field

内分泌・代謝