RhoF promotes murine marginal zone B cell development

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591416
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: KATSUMI Akira 浜松医科大学, 医学部, 准教授 (80378025)
• Co-Investigator(Kenkyū-buntansha): MATSUSHITA Tadashi 名古屋大学, 医学部附属病院, 教授 (30314008) ; AMANO Mutsuki 名古屋大学, 大学院医学系研究科, 准教授 (90304170)
• Co-Investigator(Renkei-kenkyūsha): MARUYAMA Mitsuo 国立長寿医療研究センター研究所, 老化機構研究部, 部長 (00212225)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: RhoF / filopodia / marzinal zone / knock out

Outline of Final Research Achievements

RhoF is a member of the Rho GTPase family that has been implicated in various cell functions including long filopodia formation. Although RhoF is expressed in lymphoid tissues, the roles of RhoF in B cell development remain largely unclear. We analyzed B cell development in RhoF knockout (KO) mice and found a significant reduction in marginal zone (MZ) B cells in the spleen, although T cell development in the thymus and spleen was not affected. Consistent with these results, the width of the MZ B cell region in the spleen was significantly reduced in the RhoF KO mice. However, the antigen-specific antibody titer of IgM and IgG3 after MZ B cell-specific antigen (T cell-independent antigen, type I) stimulation was not affected by RhoF deletion. Furthermore, we demonstrated that RhoF was dispensable for stromal cell-derived factor-1α- and B lymphocyte chemoattractant-induced B cell migration. These results suggest that RhoF promotes MZ B cell development in the spleen.

Free Research Field

血液・免疫