Development of a novel antileukemia adoptive immunotherapy using leukemia-reactive gene-modified memory T cells.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591426
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Ehime University
• Principal Investigator: FUJIWARA HIROSHI 愛媛大学, 医学部附属病院, 講師 (20398291)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 遺伝子細胞免疫療法 / T細胞受容体（TCR）遺伝子 / 遺伝子改変Tリンパ球 / 免疫記憶細胞 / 白血病幹細胞

Outline of Final Research Achievements

The clinical efficacy mediated by gene-modified CD8+ T-lymphocytes using leukemia antigen-specific T-cell receptor (TCR) gene transfer (artificial cytotoxic T lymphocytes: artificial CTL) depends on the in vivo persistence of those artificial CTL after recognition of leukemia cells. In this study, to this end, we assessed the helper function mediated by newly gene-modified CD4+ T-lymphocytes (artificial helper T cells) using the identical HLA class I-restricted TCR gene which was employed for artificial CTLs. In results, those artificial helper T cells successfully stimulated artificial CTLs to survive longer after recognition of leukemia cells in vivo through the facilitation of memory T-cell formation mediated by artificial CTL.

Free Research Field

血液内科学、腫瘍免疫、造血幹細胞移植、感染免疫