2014 Fiscal Year Final Research Report
Basic research for hematopoietic stem cell transplantation before birth
| Project/Area Number |
24591429
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Research Collaborator |
NAGAO Yosikazu 宇都宮大学, 農学部, 教授 (70291953)
ABE Tomoyuki 自治医科大学, 医学部 分子病態治療研究センター, 助教 (20610364)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 造血幹細胞移植 / 出生前治療 / 先天性免疫不全症 / 大型動物 / ヒツジ / 子宮内移植 |
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| Outline of Final Research Achievements |
X-linked severe combined immunodeficiency has been successfully treated in US and European countries by in utero transplantation (IUT) of hematopoietic stem cells; however, the engraftment efficiency has not necessarily been high enough for other diseases in which graft cells do not have growth advantage. From a series of experiments in the setting of sheep IUT, we have shown that the engraftment efficiency can be improved by the following methods: (1) Pre-conditioning of fetuses with busulfan, (2) transient forced expression of the HoxB4 gene in donor hematopoietic stem cells, and (3) post-conditioning with cytokines.
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| Free Research Field |
再生医学、血液学
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