Metabolomic analysis in IgG4-related disease

2015 Fiscal Year Final Research Report

• Project/Area Number: 24591459
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Kanazawa Medical University
• Principal Investigator: IWAO Haruka 金沢医科大学, 医学部, 助教 (10612244)
• Co-Investigator(Kenkyū-buntansha): UMEHARA Hisanori 金沢医科大学, 医学部, 教授 (70247881) ; KAWANAMI Takafumi 金沢医科大学, 医学部, 講師 (20350762) ; MASAKI Yasufumi 金沢医科大学, 医学部, 教授 (40238895) ; TANAKA Masao 京都大学, 医学部, 特定准教授 (10332719)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: IgG4関連疾患 / メタボロミクス / 脂質メディエーター

Outline of Final Research Achievements

IgG4-related disease is indicated by increased infiltration of IgG4-positive plasma cells, typically elevated IgG4 serum levels and the formation of lymphoplasmacytic infiltrate in one or more organs. We explored the metabolome of >400 small molecules in serum from 10 matched pair IgG4-related disease subjects prior to (PRE) and following (POST) steroid treatment and contrast these profiles with a cohort of 10 normal non-diseased subjects (NORM). The polyunsaturated fatty acid arachidonate is involved in pro-inflammatory activities through its metabolism to the pro-inflammatory prostaglandins via cyclooxygenase activity and HETEs via lipoxygenases. Individuals with PRE IgG4-related disease had elevated arachidonate and the 12-lipoxygenase product, 12-HETE, as compared to NORM subjects. The higher levels of the pro-inflammatory arachidonate and 12-HETE was unaffected by steroid treatment as POST subjects had similar levels of these biochemicals as was observed in PRE subjects.

Free Research Field

膠原病学、血液学