Study on the bactericidal effect of chlorine dioxide against bacterial spores.

2015 Fiscal Year Final Research Report

• Project/Area Number: 24591494
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Kawasaki Medical School
• Principal Investigator: Yamada Sakuo 川崎医科大学, 医学部, 准教授 (00122458)
• Co-Investigator(Kenkyū-buntansha): Ohge Hiroki 広島大学, 医学部, 教授 (70379874)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 芽胞 / 消毒薬 / 二酸化塩素 / 次亜塩素酸ナトリウム / 電子顕微鏡

Outline of Final Research Achievements

The bactericidal activity of chlorine dioxide (ClO2) against Bacillus cereus spores was evaluated using the viable cells count method. The results demonstrated that the number of viable cells was decreased to approximately 1/1000 following 1 min of treatment . In contrast, In contrast, no bactericidal effect was observed when the spores were treated for 15 min with a 0.02% (w/v) solution of sodium hypochlorite (NaOCl), suggesting that ClO2 was more effective in terms of its sporicidal effect than NaOCl. When the spores treated with NaOCl were observed by scanning electron microscopy and transmission electron microscopy, it was clear that the coats of these spores had been partially damaged by the treatment process, whereas no significant morphological changes were induced by the ClO2 treatment, even at a high concentration of ClO2. These morphological analyses suggested that the mechanism of the bactericidal activity of ClO2 towards B. cereus spores was different from that of NaOCl.

Free Research Field

微生物学