Live imaging analysis of cutaneous immune responses

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591650
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kyoto University
• Principal Investigator: HONDA Tetsuya 京都大学, 医学(系)研究科(研究院), 准教授 (40452338)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: アトピー性皮膚炎 / 皮膚樹状細胞 / 脂質メディエーター

Outline of Final Research Achievements

Resolvin E1 (RvE1) is a lipid mediator derived from ω3 polyunsaturated fatty acids that exerts potent anti-inflammatory roles in several murine models. Anti-inflammatory mechanism of RvE1 in acquired immune responses has been attributed to attenuation of cytokine production by dendritic cells (DCs). In this study, we newly investigated the effect of RvE1 on DC motility using a contact hypersensitivity (CHS) model and found that RvE1 impaired DC motility in the skin using two-photon microscopy. RvE1 attenuated T cell priming in the draining lymph nodes and effector T cell activation in the skin, leading to the reduced skin inflammation in CHS. On the other hand, leukotriene B4 (LTB4) induced actin filament reorganization in DCs and increased DC motility by activating Cdc42 and Rac1 via BLT1, which was abrogated by RvE1. Our results suggest that RvE1 attenuates cutaneous acquired immune responses by inhibiting cutaneous DC motility possibly through LTB4-BLT1 signaling blockade.

Free Research Field

皮膚科学