Elucidation of pathologic conditions and establishment of the diagnostic methods in hydrolyzed wheat protein (Glupearl: GP19S)- induced immediate-type wheat allergy.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591671
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Fujita Health University
• Principal Investigator: MATSUNAGA Kayoko 藤田保健衛生大学, 医学部, 教授 (80131192)
• Co-Investigator(Kenkyū-buntansha): YAGAMI Akiko 藤田保健衛生大学, 医学部, 准教授 (90367699) ; SANO Akiyo 藤田保健衛生大学, 医学部, 助教 (20625983) ; NAKAMURA Masashi 藤田保健衛生大学大学院, 医学研究科 (30449467)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 加水分解コムギ / グルパール１９S / 即時型コムギアレルギー / 特異IgE抗体 / 検査法 / 抗原解析 / 交叉反応性 / 予後因子の探索

Outline of Final Research Achievements

The purpose of this study was to elucidate the pathologic conditions and to establish the diagnostic methods in hydrolyzed wheat protein- induced immediate-type wheat allergy (HWP-IWA) . Our findings show that the ELISA-based GP19S specific IgE assay is a useful quantitative and high throughput method for the diagnosis. Using ELISA, skin prick test and Western blotting, we elucidated that patients who developed an allergy to GP19S also had cross-reactivity to some other HWPs. This suggested that other HWPs might contain the epitope sequences in GP19S. On the other hands, the GP19S antigen was thought to be generated after gluten was exposed to hydrochloric acid and heated to 95 °C. The heating time was long enough to initiate deamidation but too short to initiate a change in molecular weight, and precipitation at the isoelectric point resulted in an increase in antigen concentration.

Free Research Field

アレルギー疾患研究