Preclinical PET imaging for the daignosis and treatment of pancreatic tumor model

2015 Fiscal Year Final Research Report

• Project/Area Number: 24591804
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: Aung U.Winn 国立研究開発法人放射線医学総合研究所, 分子イメージング研究センター, 主任研究員 (70392214)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 膵臓癌 / 同所移植癌モデル / 核医学イメージング（PETを含む）

Outline of Final Research Achievements

The purpose of our study is to develop a clinically relevant orthotopic xenotransplantation model of pancreatic cancer that reflects the clinical situation and, to perform the preclinical evaluation of new diagnostic and therapeutic PET imaging probes, allowed in this model. At first, we succeeded to establish the orthotopic pancreatic cancer xenograft model. Next, among three candidates of PET imaging probes (64Cu-RAFT-RGD, 11C-Sorafenib, 11C-DAC), we put focus on the 64Cu-RAFT-RGD probe and performed in vitro and in vivo experiments, small animal 64Cu-RAFT-RGD PET imaging comparing with 18F-FDG-PET, contrasted CT (CECT) after PET, and examined the potential applicability of probe. Postimaging autoradiography, histologic, immunohistochemical and immunofluorescence examinations, and the comprehensive results suggested that 64Cu-RAFT-RGD possesses the specific binding to αvβ3 integrin of pancreatic tumor cells and may provide higher detectability over 18F-FDG-PET.

Free Research Field

医歯薬学, 放射線医学, 放射線科学