Evaluation of cell-killing effects within solid tumors by the non-invasive real-time imaging system for hypoxic cell apoptosis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591849
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Kitasato University
• Principal Investigator: KAGIYA Go 北里大学, 医療衛生学部, 講師 (30524243)
• Co-Investigator(Kenkyū-buntansha): OGAWA Ryohei 富山大学大学院, 医学薬学研究部, 准教授 (60334736)
• Co-Investigator(Renkei-kenkyūsha): MATSUMOTO Hideki 福井大学, 高エネルギー医学研究センター, 准教授 (40142377)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 低酸素細胞 / アポトーシス / 非侵襲的リアルタイムイメージング / 可視化

Outline of Final Research Achievements

The presence of hypoxic regions within solid tumors is caused by an imbalance between cell proliferation and angiogenesis. Such regions may facilitate the onset of recurrence after radiation therapy and chemotherapy, as hypoxic cells show resistance to these treatments. The non-invasive real-time imaging system to monitor hypoxic cell apoptosis is considered to be useful system for screening and evaluation of therapeutic approaches targeting the hypoxic cells. We thus started developing the imaging system for hypoxic cell apoptosis. The imaging system in which cyclic luciferase gene was expressed under the control of the hypoxic responsive promoter has successfully made it possible to visualize real-time hypoxic cell apoptosis in vitro.

Free Research Field

放射線生物学