Research on new mechanism for radioprotective effect of FGF12

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591856
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: NAKAYAMA Fumiaki 独立行政法人放射線医学総合研究所, 重粒子医科学センター, チームリーダー (50277323)
• Co-Investigator(Renkei-kenkyūsha): IMAI Takashi 独立行政法人放射線医学総合研究所, 重粒子医科学センター, プログラムムリーダー (50183009)
• Research Collaborator: UMEDA Sachiko
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 放射線障害 / 防護剤 / 治療薬 / 増殖因子

Outline of Final Research Achievements

FGF12 can be internalized into cells using two domains (CPP-M, CPP-C).　This study aimed at clarifying the role of FGF12 internalization in protection against radiation-induced intestinal injury. Administration of FGF12 into BALB/c mice after radiation exposure was as effective as pretreatment in significantly promoting intestinal regeneration from radiation damage. Two domains, comprising amino acid residues 80-109 and 140-169 of FGF12B, were identified as being responsible for the radioprotective activity. Interestingly, these regions included the CPP-M and CPP-C domains, respectively; however, CPP-C by itself did not show an anti-apoptotic effect. In addition, internalized FGF12 suppressed the activation of p38α after irradiation, resulting in reduced radiation-induced apoptosis. These findings indicate that FGF12 can protect the intestine against radiation-induced injury through its internalization, independently of FGFRs.

Free Research Field

放射線科学