Strategy to overcome chemoresistance of cancer stem cells by regulating cancer microenvironment and intracellular redox state

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591965
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: The Tazuke Kofukai
• Principal Investigator: UEDA Shugo 公益財団法人田附興風会, 医学研究所 第1研究部, 主任研究員 (80372580)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 癌幹細胞 / CD44 / レドックス / 抗癌剤感受性 / 消化器癌 / ガレクチン

Outline of Final Research Achievements

Gastrointestinal cell lines expressing several amount of CD44, an important marker for cancer stem cell, were investigated. There was no difference in susceptibility of hydrogen peroxide-induced cell death and intracellular reactive oxygen species (ROS) between normoxia and hypoxia in HCT116 and HT-29 that express higher levels of CD44. All-trans retinoic acid, a clinically effective inducer of differentiation in acute promyelocytic leukemia cells, induced cell death by increasing intracellular ROS but had no effect on differentiation in these cell lines.
Expression of galectin-7 was lower in cancer cells than non-cancer tissues in clinical esophageal cancer. The epigenetic regulation of galectin-7 expression is under investigation.

Free Research Field

医歯薬学