2014 Fiscal Year Final Research Report
Development of novel herpes virus therapy on colorectal cancer
| Project/Area Number |
24591969
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAZAWA Kentaro 東京大学, 医科学研究所, 助教 (60572542)
TSURITA Giichiro 東京大学, 医科学研究所, 講師 (80345206)
HATA Keisuke 東京大学, 医科学研究所, 助教 (60526755)
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| Co-Investigator(Renkei-kenkyūsha) |
TODO Tomoki 東京大学, 医科学研究所, 教授 (80272566)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | がん / ウイルス療法 |
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| Outline of Final Research Achievements |
G47delta is a modified herpes simplex virus type 1, which selectively proliferate in only tumor cells and destroy the host cell, and seldom affect non-tumor cells. We aimed to evaluate clinical effect of G47delta on colorectal cancer and performed a pre-clinical study. A cytotoxic assay revealed statistically significant cytotoxicity on cell lines compared with the control, according to the concentration of the virus and the term of the treatment. We confirmed that the virus was incorporated in the tumor cell though X-gal staining assay. Replication assay was performed to investigate proliferation of the virus in the cell lines, and we affirmed it. We made subcutaneous tumors of the cell lines. After injection of the virus to the tumors, we found suppression of tumor growth compared with the control.
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| Free Research Field |
消化器外科
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