2014 Fiscal Year Final Research Report
Establishment of cancer antigen-specific CTL therapy by a concomitant functional regulation of CTL and regulatory T cell and an inhibition of T cell differentiation
| Project/Area Number |
24591973
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TANI Tohru 滋賀医科大学, 医学部, その他(特任教授) (20179823)
KURUMI Yoshimasa 滋賀医科大学, 医学部, 教授 (70205219)
SHIMIZU Tomoharu 滋賀医科大学, 医学部, 助教 (70402708)
MIYAKE Tohru 滋賀医科大学, 医学部, その他(客員助教) (70581924)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 腫瘍免疫 / 免疫治療 / 癌ワクチン / T細胞補助刺激 / 細胞治療 / 養子免疫 / CTL |
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| Outline of Final Research Achievements |
We have done the research to develop the efficient cancer-specific CTL therapy, which was to generate CTLs ex vivo and to transfer them into patients.
Cancer-specific CTLs were efficiently generated when T cells were cultured with a cancer antigen and OX40 costimulation that could enhance the effector T cell function. When these cancer-specific CTLs were adoptively transferred into the tumor-bearing mice pretreated with OX40 costimulation that could also inhibit the regulatory T cell function, CTLs could overcome immune tolerance condition, maintain its function, and finally eliminate tumor. IL-2 and/or B7-DC proved to be important molecules that could contribute to the generation of effective CTLs.
Human lymphocytes from the volunteers could generate antigen (HA)-specific CTLs when cultured with an antigen and OX40 stimulation. From these findings, we believe that cancer-specific CTL therapy treated with costimulatory molecules can be applied in a clinical setting in future.
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| Free Research Field |
外科腫瘍学、腫瘍免疫、
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