Predictive diagnostics effect and clinical application of circulating cancer stem-like cells in colon cancer patients treated with molecular targeted therapy

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591984
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Teikyo University
• Principal Investigator: IINUMA HISAE 帝京大学, 医学部, 講師 (30147102)
• Co-Investigator(Kenkyū-buntansha): WATANABE Toshiaki 東京大学, 医学部, 教授 (80210920) ; YAMADA Hideki 帝京大学, 医学部, 助手 (50525269) ; HAYAMA Tamuro 帝京大学, 医学部, 助手 (80533744)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 循環腫瘍細胞 / 癌幹細胞 / 大腸癌 / 転移診断 / 予後予測

Outline of Final Research Achievements

Recently, we make clear that the some population of CTCs expressing the cancer stem cells marker, and named these cells as circulating tumor stem cell (CTSC). Here we aimed to clarify the clinical significance of CTSC and CTC (CTSC /CTC) in colorectal cancer patients. Multi-marker panel consist of CEA, epithelial markers (CK19, CK20), cancer stem cell marker (CD133, CD44) and new marker X were used for detection of CTSC/CTC.
In this study, we demonstrated that CTSC/CTC level was a significant prognostic factor for OS and DFS in patients with Dukes stage B and C cancer. Next, we assessed the value of CTSC/CTC levels as biomarkers for clinical response in advanced CRC patients treated with molecular target reagent (bevacizumab, cetuximab) and chemotherapy (FOLFOX, FOLFIRI). We demonstrated that CTSC/CTC levels of PB have a predictive value for clinical response and survival in patients treated with these combination therapies.

Free Research Field

外科腫瘍学